endo-1,4 β-Mannanase （T. maritima） EC 3.2.1.78 CAZY Family: GH5 From Thermotoga maritima. Electrophoretically homogeneous. In 3.2 M ammonium sulphate. Specific activity: 22.9 U/mg （80°C, pH 7.0, carob galactomannan as substrate）. Contaminants: β-mannosidase and α-galactosidase< 0.004%. Stable at 4°C for > 2 years.
Content: 250 U at 80oC
Appearance Liquid suspension Specific Gravity ~ 1.5 Solubility in Water Readily soluble pH Value 6.0 Odour None Form Liquid Stability Stable in a refrigerator for five or more years Ingredients Name CAS Proportion b-Mannanase （Thermotoga maritima） 1% Ammonium Sulphate 45% Sodium Azide 0.02% Water 54%
PROPERTIES 1. ELECTROPHORETIC PURITY - Single band on SDS-gel electrophoresis （MW ~ 75,370） - Single major band on Isoelectric focusing （pI ~ 5.1） 2. SPECIFIC ACTIVITY 22.9 U/mg protein at pH 7.0 and 80°C. One Unit of b-mannanase activity is defined as the amount of enzyme required to release one μmole of mannose-reducing-sugar equivalents per minute from low viscosity carob galactomannan （6.25 mg/mL） in 50 mM MOPS buffer （pH 7.0） at 80°C. 3. RELATIVE RATES OF HYDROLYIS OF SUBSTRATES SUBSTRATE RELATIVE HYDROLYSIS RATES Carob galactomannan （low viscosity） 100 CMC-4M 3.67 p-NP-a-D-galactoside < 0.45 p-NP-b-D-glucoside < 0.45 Activities on polysaccharides were determined at a final substrate concentration of 6.25 mg/mL in the reaction mixture in 50 mM MOPS buffer （pH 7.0） at 40°C using the Nelson/Somogyi reducing sugar procedure. Action on p-NP-substrates was determined at a final substrate concentration of 5 mM in 100 mM MOPS buffer （pH 7.0） at 40°C. 4. PHYSICOCHEMICAL PROPERTIES pH Optimum: 7.0 （at 40°C） Temperature Optimum: 85°C pH Stability: 6.0-8.0 （20 hr at 4°C） Temperature Stability: unstable above 80°C （15 min at pH 7.0） 5. STORAGE CONDITIONS The enzyme is supplied as an ammonium sulphate suspension in 0.02 % （w/v） sodium azide and should be stored at 4°C. Swirl to mix the enzyme immediay prior to use.
